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008090960861785008646234
pht
 pht      22.07.2019 - 12:02:02 , level: 1, UP   NEW
How biologists are creating life-like cells from scratch
https://www.nature.com/articles/d41586-018-07289-x
http://buildacell.io/
http://buildacell.io/engineering/build-a-cell/

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pht
 pht      27.06.2019 - 16:55:53 , level: 1, UP   NEW
https://soundcloud.com/a16z/damage-free-genome-editing-next-in-crispr-recent-journal-papers

Two recent scientific journal papers show what’s possible when CRISPR moves from cutting DNA tool to a full-fledged platform — expanding its toolkit for medicine across R&D, therapeutics, and diagnostics:

“Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration” in Nature — by Sanne Klompe, Phuc Vo, Tyler Halpin-Healy, and Samuel Sternberg (of Columbia University)

“RNA-guided DNA insertion with CRISPR-associated transposases” in Science — by Jonathan Strecker, Alim Ladha, Zachary Gardner, Jonathan Schmid-burgk, Kira Makarova, Eugene Koonin, and Feng Zhang (of the Broad Institute)

What do these two papers — both about techniques for getting rid of the need to cut the genome to edit it — make possible going forward, given the ongoing shift of biology becoming more like engineering? Where are we in the wave of the genome engineering “developer community” building on top of CRISPR with a constantly growing suite of programmable functionalities? a16z bio general partner Jorge Conde and bio deal team partner Andy Tran chat with Hanne Tidnam about these trends — and these two papers — in this short internal hallway-style conversation, part of our new a16z Journal Club series.

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pht
 pht      19.06.2019 - 19:22:32 , level: 1, UP   NEW
Illustrated Guide to Home Biology Experiments: All Lab, No Lecture (DIY Science)

lrg.jpg

Experience the magic of biology in your own home lab. This hands-on introduction includes more than 30 educational (and fun) experiments that help you explore this fascinating field on your own. Perfect for middle- and high-school students and DIY enthusiasts, this full-color guide teaches you the basics of biology lab work and shows you how to set up a safe lab at home.

The Illustrated Guide to Home Biology Experiments is also written with the needs of homeschoolers firmly in mind, as well as adults who are eager to explore the science of nature as a life-long hobby. To get the most from the experiments, we recommend using this guide in conjunction with a standard biology text, such as the freely downloadable CK-12 Biology (ck-12.org).

Master the use of the microscope, including sectioning and staining
Build and observe microcosms, soda-bottle worlds of pond life
Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA
Extract, isolate, and observe DNA
Explore photosynthesis, osmosis, nitrogen fixation, and other life processes
Investigate the cell cycle (mitosis and cytokinesis)
Observe populations and ecosystems, and perform air and water pollution tests
Investigate genetics and inheritance
Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection
Gain hands-on lab experience to prepare for the AP Biology exam
https://www.thehomescientist.com/manuals/Illustrated_Guide_to_Home_Biology_Experiments.pdf

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pht
 pht      13.06.2019 - 12:22:47 [7K] , level: 1, UP   NEW
uplne prve helloworld pokusy na agare

vzorka z drazdiaku po 1 dni:
3yOLaQL.jpg

po tyzdni+:
gb0lH9x.jpg

37GmXAh.jpg
aspon 5 roznych kolonii


odtlacok zubnej kefky po 3 dnoch:
ssgn9Uw.jpg

asi vydareny klon bez kontaminacie
4ZRdaW8.jpg

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blba zena
 blba zena      22.07.2019 - 20:28:54 (modif: 22.07.2019 - 20:32:28), level: 2, UP   NEW !!CONTENT CHANGED!!
neviem ake medium pouzivas (predpokladam ze variant domaceho LB), ale Drazdiak aj ine priklady mozu byt uplne nevypovedne, lebo kopa mikroorganizmov nie je tak jednoducho kultivovatelna a potrebuju specialnejsie podmienky

niekde by som mala mat pdfko s najcastejsimi mikroorganizmami, ktore pripravil Virologicky ustav na air sampling pri Noci vyskumnikov, zopar veci co ti vyrastlo by si tam urcite identifikoval :)

edit:
air-sampling-popis-Noc-vyskumnikov.jpg

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pht
 pht      22.07.2019 - 11:43:11 [2K] , level: 2, UP   NEW
drazdiak po dalsom tyzdni:
HGxXVcI.jpg?1

po dychnuti a +1 tyzdni:
j8C0u2Q.jpg

uz zdrha:
Chxy0sX.jpg?1

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jonas
 jonas      22.07.2019 - 14:15:59 (modif: 22.07.2019 - 14:16:11), level: 3, UP   NEW !!CONTENT CHANGED!!
my sme skusili 2 days ago, uz je tam prvy feši

M1TJ6EG.jpg

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milton
 milton      14.06.2019 - 11:42:23 , level: 2, UP   NEW
Na čo sa to pozerám?
Petriho misku poznám. :-)

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pht
 pht      14.06.2019 - 14:35:19 [2K] , level: 3, UP   NEW
neviem na co sa ty pozeras, ale na obrazku su nejake random neidentifikovane bakterialne kolonie a mycelium z kusku hypsizygus tessellatus

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lakerko
 lakerko      14.06.2019 - 08:40:31 , level: 2, UP   NEW
TO POSLEDNE VYZERA AKO PENIS S VAJCAAAAMI!!!

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lazyShepherd
 lazyShepherd      14.06.2019 - 07:45:57 , level: 2, UP   NEW
O co ide? :) Teda ... napises viac?

~ What a time to be alive... ~

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pht
 pht      14.06.2019 - 14:47:36 , level: 3, UP   NEW
ide len o zvedavost, prve pokusy ako narabat s miskami, pri tom drazdiaku a kefke to bolo jedno, ale napriklad pri klonovani huby z obchodu sa kontaminovalo 3 zo 4 misiek aj tak ze som dokladne dezinfikoval a robil v still air boxe

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darmozrac
 darmozrac      15.06.2019 - 07:18:02 , level: 4, UP   NEW
myslim ze na taketo veci by bol lepsi flow box (laminar), videl som aj nejake tutorialy ako si to doma vyrobit

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pht
 pht      15.06.2019 - 09:30:38 , level: 5, UP   NEW
viem pozeral som a zvazujem ale kvoli hepa filtru je to radovo nakladnejsie aj diy, SAB ma vysiel <10e a hodinu casu, da sa s tym asi tiez len to nie je ideal

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stamina
 stamina      14.06.2019 - 00:41:08 [9K] , level: 2, UP   NEW
uz ta vidim, jak to zeres bez chleba!

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pht
 pht      10.06.2019 - 18:04:40 [1K] , level: 1, UP   NEW
https://www.appropedia.org/Hackteria
https://www.hackteria.org/wiki/Main_Page

Hackteria | Open Source Biological Art, DIY Biology, Generic Laboratory Infrastructure

Hackteria is an international network active since 2009 in the field of Open Source Biological Art. As a community platform hackteria tries to encourage the collaboration of scientists, hackers and artists to combine their experitise, write critical and theoretical reflections, share simple instructions to work with lifescience technologies and cooperate on the organization of workshops, temporary labs, hack-sprints and meetings. Hackteria is a network of people practicing DIY (do-it-yourself) and DIWO (do-it-with-others) biology with an interest in art, design and interdisciplinary cooperation. Hackteria operates on a global scale, and is based on a web platform and a wiki for sharing knowledge, which enable anyone to learn but also test different ways of hacking living systems. Hackteria is not based in a physical space, and its goal is to allow artists, scientists and hackers to collaborate and test various biohacking and bioart techniques outside the official laboratories and art institutions, basically anywhere in the world.

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jonas
 jonas      11.06.2019 - 12:54:45 , level: 2, UP   NEW
akurat som vcera pozeral toto: http://hackteria.org/wiki/index.php/Algae_Culture_at_Home
cisticky vzduchu <3

008090960861785008632654
pht
 pht      05.06.2019 - 16:33:54 [1K] , level: 1, UP   NEW
1*RLrER75EWAIUsrsoNosLGQ.jpeg

A Guide to DIYbio (updated 2019)

008090960861785008632653
pht
 pht      05.06.2019 - 16:31:45 , level: 1, UP   NEW
https://diybio.org/
https://sphere.diybio.org/

DIYbio.org was founded in 2008 with the mission of establishing a vibrant, productive and safe community of DIY biologists. Central to our mission is the belief that biotechnology and greater public understanding about it has the potential to benefit everyone.

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SYNAPSE CREATOR
 pht      31.05.2019 - 20:04:51 [1K] , level: 1, UP   NEW  HARDLINK
https://pechblenda.hotglue.me/?transhackfeminism_en/
https://we.riseup.net/gynepunklab

Pechblenda lab was born out of the necessity to generate a space in Calafou (a community in a large former industrial space) for us to flourish, a non-patriarchal TransHackFeminist space where free knowledge springs from raw experimentation (electronic repairs, experiments with turbines, bioelectrochemistry, sound .... ) and self education.

Sick of the filthy dust, montonous and boring, of stagnant, unbreathable, competitive and excluding environments, of semi-free information which is actually totally controlled, power and decision of hunched up egocentric and infantile machos. Tired of repressed, impenetrable and homogenous bodies, we are resetting and migrating our bodies, modificable codes, lubricated and fluid, far from this sad landscape.

Tired of the useless and recursive manipulation of information, we study, construct and fail with all that is around us, with multiple, monstruous and hateful ends. From the expansion of information to the mutation of dispositives, we want to hack and recodify everything that is static and programmed by social and technological imposition.

PECHBLENDA is injected into our veins as an antidote to the heteropatriarchal arrogance that surrounds us. A disturbance, a transhackerfeminist electronic distortion.

We have found the place for our rituals,
we had dreamed it, written it in science fiction.

Now we live it with high voltage potentiality,
with the intensity of the shadows,
taking off together with desires in common,
with our differences.

The walls tremble and the water penetrates the tiny holes,
it expands like an unbreakable code exciting our neurons ;
we change the apparent path of events transiting antimelodies,
noise as arithmetic opening, outside of the calculated and homogenous,
noise feeding unlimited experimentation.

If we cant make noise its not our revolution.

Improvised performance creating and breaking codes, constructing hybrid machines.
Beat roots and obscure mutant landscapes that become the uncontrollable secretions of our desires. Electronautics and bioelectricity that chemically saturate the environment,
the acid smell of our hormones shakes the space,
resituated amongst cables, resistences, condensors and corrosive liquids.

Nature and technology are not different,
nature was to the witches what technoscience is to us, the cyborg witches.
We infiltrate the machine with our hands, sweat and disperse attention,
we prepare ourselves for inexact verification where the apparent error is desired,
where we fail, fuck, we are.

We are geek whores,
cyborg bitches.
We devour Haraway and Asimov,
Preciado and Python manuals,
Itziar Ziga and Neil Stephenson,
Margulis and Despentes,
hackmeetings and transfeminist workshps,
DIY electronics and sexual bricolage ;
we absorb PDFs of electronics theory y listen to psicofonias from around :
we read and design circuits,
and experiment with them in our bodies.

We scream noise and cyborg covens,
soldering and alchemy,
we spit out performances and install gnu-linux,
we love recycling and reparing with our breasts bared.

We laugh about everything, about ourselves ..
we detest the politically correct.
We parody what is socially understood to be feminine, what is supposed to be masculine.
We question the identity of assigned genders,
we exagerate it, ridiculise it.
Extremely sexual, ironic, sarcastic,
we love to party, to not sleep,
to take drugs if we feel like it,
to go with our friends
or to finish a circuit
or improvise an eternal noise jam.

Fed by pornoterrrorism and free culture,
we know how to use our claws and teeth if needs be.

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pht
 pht      29.05.2019 - 19:43:01 , level: 1, UP   NEW
https://brilliant.org/courses/computational-biology/

Computational biology merges the algorithmic thinking of the computer scientist with the problem solving approach of physics to address the problems of biology. Since the year 2000, an ocean of sequencing data has emerged that allows us to ask new questions.

Here we'll develop intuition for a selection of foundational problems in computational biology like genome reconstruction, sequence alignment, and building phylogenetic trees to look at evolutionary relationships. We also address certain physicochemical problems of molecular biology like RNA folding.
https://www.edx.org/course/principles-of-synthetic-biology

Do you like biology, biotechnology, or genetic engineering? Are you interested in computer science, engineering, or design? Synthetic Biology is an innovative field bringing together these subject areas and many more to create useful tools to solve everyday problems.

This introductory synthetic biology course starts with a brief overview of the field and then delves into more challenging yet exciting concepts. You will learn how to design your very own biological regulatory circuits and consider ways in which you can apply these circuits to real-world problems we face today.

From basic oscillators, toggle switches, and band-pass filters to more sophisticated circuits that build upon these devices, you will learn what synthetic biologists of today are currently constructing and how these circuits can be used in interesting and novel ways.

Join us as we explore the field of synthetic biology: its past, present, and promising future!

What you'll learn
Modern techniques in DNA assembly and regulation of gene expression and protein activity
How to design basic biological circuits
Principles for scaling up and creating large-scale biological circuits and regulatory networks
Basic ODE modeling of biological systems with MATLAB, introduction to Cello: genetic circuit design automation software

008090960861785008626645
pht
 pht      19.05.2019 - 21:12:30 , level: 1, UP   NEW
primer for synthetic biology (2007 draft)
https://s3-us-west-2.amazonaws.com/oww-files-public/3/3d/SB_Primer_100707.pdf

“Synthetic biology,” in the modern sense, means using engineering
principles to create functional systems based on the molecular machines and
regulatory circuits of living organisms. However, it also includes going beyond
them to develop radically new systems. At the very least, synthetic biology
represents a merger of molecular biology, genetic engineering and computer
science1
– and given the scale of the components that it works with, it also
qualifies as a form of nanotechnology. Students and others who wish to learn
about or – better still – to do synthetic biology often approach this exciting field
with only some of the background necessary to grasp its essentials. This threepart primer represents an effort to assist them in filling gaps in their knowledge.
Part I, “Molecular Biology for Novices,” summarizes the key aspects of
biochemistry and molecular biology that bear on synthetic biology. Part II,
“Engineering for Biologists,” introduces some basic engineering principles and
the most important tools used in synthetic biology. Part III, “Ethics for
Everyone,” briefly outlines the key ethical issues facing synthetic biology today.

008090960861785008626641
pht
 pht      19.05.2019 - 20:58:08 , level: 1, UP   NEW
https://waag.org/en/labs/open-wetlab
https://www.youtube.com/channel/UCVzZljBZA1AjfWBTrJuMSzA/videos

The Open WetLab is a leading place for bio-art, biodesign and do-it-together biology in the heart of Amsterdam. It works together with artists, designers, scientists, and hackers at public participation projects.

The Lab regularly organizes well-visited meet-ups. Beside that, artists and students can work in the lab as artistic researcher-in-residence and new biohackers are trained at the BioHack Academy: an international highly acclaimed course for biohackers, initiated by Pieter van Boheemen. The BioHack Academy learns individuals to build their own lab, create bio experiments, and teaches the basics of synthetic biology and critical reflection.
http://biohackacademy.github.io/

In BioHack Academy you design, build, use and share your own biolab. Each edition connects several groups around the world passionate about biotech and hacking.

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pht
 pht      16.05.2019 - 23:29:39 (modif: 16.05.2019 - 23:30:07) [6K] , level: 1, UP   NEW !!CONTENT CHANGED!!
pR1eaBl.jpg

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niko
 niko      17.05.2019 - 15:21:16 , level: 2, UP   NEW
toto som videl jak decko jebnute a mal som nocne mory asi pol roka

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blurec
 blurec      17.05.2019 - 23:25:58 , level: 3, UP   NEW
ja som to zazil s Moontrap. Nasi mi potom nechceli dovolit pozerat ani Indiana Jones a chram skazy, pretoze sa bali, ze to zasa spusti dlhe obdobie nespavych a prestrasenych noci.

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randlover
 randlover      17.05.2019 - 23:49:52 , level: 4, UP   NEW
to si musím pozrieť. ku mne sa dostala kazeta s filmom necronomicon. ani som to nedopozeral, zosratý som bol až za ušami

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blurec
 blurec      17.05.2019 - 23:53:26 , level: 5, UP   NEW
to je skvela pripomienk! uz dlhsie sa pohravam s myslienkou zacat zaradom pozerat filmy s Jeffrey Combs...

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aufhebung
 aufhebung      17.05.2019 - 19:45:46 (modif: 17.05.2019 - 19:58:38) [2K] , level: 3, UP   NEW !!CONTENT CHANGED!!
ma to paradne prakticke efekty uplne nunu a pritom lovestory

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:):
 :):      17.05.2019 - 12:04:51 , level: 2, UP   NEW
To je z čoho

アoレム尺 oアアo丂ノイノo刀

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majkl
 majkl      17.05.2019 - 12:09:04 [1K] , level: 3, UP   NEW
https://www.csfd.cz/film/1789-moucha/prehled/

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:):
 :):      17.05.2019 - 12:25:07 , level: 4, UP   NEW
Nice diky

アoレム尺 oアアo丂ノイノo刀