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ID 1085352[L...2
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008090960861785008687310
SYNAPSE CREATOR
 pht      05.12.2019 - 17:46:40 , level: 1, UP   NEW  HARDLINK
In vivo production of psilocybin in E. coli
https://www.sciencedirect.com/science/article/pii/S109671761930309X

Highlights
• First example of psilocybin production in prokaryotic host.

• Genetic optimization shows high sensitivity to changes in transcriptional landscape.

• Pathway bottlenecks removed through fine-tuned optimization.

• Gram-scale production of psilocybin in Escherichia coli.


Abstract
Psilocybin, the prodrug of the psychoactive molecule psilocin, has demonstrated promising results in clinical trials for the treatment of addiction, depression, and post-traumatic stress disorder. The development of a psilocybin production platform in a highly engineerable microbe could lead to rapid advances towards the bioproduction of psilocybin for use in ongoing clinical trials. Here, we present the development of a modular biosynthetic production platform in the model microbe, Escherichia coli. Efforts to optimize and improve pathway performance using multiple genetic optimization techniques were evaluated, resulting in a 32-fold improvement in psilocybin titer. Further enhancements to this genetically superior strain were achieved through fermentation optimization, ultimIn vivo production of psilocybin in E. coliately resulting in a fed-batch fermentation study, with a production titer of 1.16 g/L of psilocybin. This is the highest psilocybin titer achieved to date from a recombinant organism and a significant step towards demonstrating the feasibility of industrial production of biologically-derived psilocybin.

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SYNAPSE CREATOR
 pht      30.11.2019 - 14:41:02 , level: 1, UP   NEW  HARDLINK
rob rhinehart zije! (po tom co si odstranil crevny mikrobiom a zahadne odisiel z internetu aj rosa labs)

Investing in the Future of Food with Rob Rhinehart and Brian Frank
https://www.spreaker.com/user/10197011/investing-in-the-future-of-food-with-rob

Brian Frank (@bfrank) of FTW Ventures, and Rob Rhinehart (@robrhinehart) of Soylent, join Erik on this episode.

They discuss:

- Some of the most exciting developments in bioscience these days.

- The possibilities for biohacking and whether there is an “AWS for bioscience” emerging.

- The interplay of startups and incumbents in the space and how incumbents can be more helpful to the startup community.

- What is under- or over-hyped in the space.

- The existing big companies in food and bioscience and what will change in the next 5-10 years.

- The future of retail and how data can transform supply chains.

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pht
 pht      20.11.2019 - 16:20:02 [1K] , level: 1, UP   NEW
v piatok 22. 11. v paralelnej polis
https://www.facebook.com/events/1473050229500557/
Rozhodli sme sa zorganizovať neformálne stretnutia v úzkom kruhu nadšencov vedy. Na stretnutiach by sme sa radi venovali rôznym témam zo sveta biotechu a microbio.
Bude to taký ten typ neformálneho rozhovoru o vede, živote a biznise pri pohári dobrého vína alebo piva (optional).
Na BioWine nájdete parťákov, s ktorými takto viete stráviť príjemný “bio” večer :)

Na to, aby si sa mohol zúčastniť nemusíš byť odborník, stačí ti túžba spoznávať. Preferenčne je však event určený pre vedcov alebo študentov microbio odborov. Tešíme sa na teba!

P.S. Zabezpečíme nejaké nápoje, ale feel free priniesť čokoľvek, čo máš rád.

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psycho
 psycho      20.11.2019 - 16:34:12 , level: 2, UP   NEW
:)

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pht
 pht      01.10.2019 - 12:37:06 , level: 1, UP   NEW
diy bio ba telegram group
https://t.me/diybiobratislava

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SYNAPSE CREATOR
 jonas      26.09.2019 - 13:28:48 , level: 1, UP   NEW  HARDLINK
Venuje sa tu niekto pestovaniu rias?

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SYNAPSE CREATOR
 pht      01.09.2019 - 16:34:32 , level: 1, UP   NEW  HARDLINK


Founders of nonfood, Lucy Chinen & Sean Raspet discuss contemporary food supply chains and sustainable food futures, including the R&D of their own algae-based nonbar. Along the way, we address: monocultures, fear, skeuomorphic flavor, cellular agriculture, and the real cost of "all-natural." (w/ Caroline Busta, Daniel Keller, @LILINTERNET)

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pht
 pht      22.07.2019 - 12:02:02 , level: 1, UP   NEW
How biologists are creating life-like cells from scratch
https://www.nature.com/articles/d41586-018-07289-x
http://buildacell.io/
http://buildacell.io/engineering/build-a-cell/

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pht
 pht      27.06.2019 - 16:55:53 , level: 1, UP   NEW
https://soundcloud.com/a16z/damage-free-genome-editing-next-in-crispr-recent-journal-papers

Two recent scientific journal papers show what’s possible when CRISPR moves from cutting DNA tool to a full-fledged platform — expanding its toolkit for medicine across R&D, therapeutics, and diagnostics:

“Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration” in Nature — by Sanne Klompe, Phuc Vo, Tyler Halpin-Healy, and Samuel Sternberg (of Columbia University)

“RNA-guided DNA insertion with CRISPR-associated transposases” in Science — by Jonathan Strecker, Alim Ladha, Zachary Gardner, Jonathan Schmid-burgk, Kira Makarova, Eugene Koonin, and Feng Zhang (of the Broad Institute)

What do these two papers — both about techniques for getting rid of the need to cut the genome to edit it — make possible going forward, given the ongoing shift of biology becoming more like engineering? Where are we in the wave of the genome engineering “developer community” building on top of CRISPR with a constantly growing suite of programmable functionalities? a16z bio general partner Jorge Conde and bio deal team partner Andy Tran chat with Hanne Tidnam about these trends — and these two papers — in this short internal hallway-style conversation, part of our new a16z Journal Club series.

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pht
 pht      19.06.2019 - 19:22:32 , level: 1, UP   NEW
Illustrated Guide to Home Biology Experiments: All Lab, No Lecture (DIY Science)

lrg.jpg

Experience the magic of biology in your own home lab. This hands-on introduction includes more than 30 educational (and fun) experiments that help you explore this fascinating field on your own. Perfect for middle- and high-school students and DIY enthusiasts, this full-color guide teaches you the basics of biology lab work and shows you how to set up a safe lab at home.

The Illustrated Guide to Home Biology Experiments is also written with the needs of homeschoolers firmly in mind, as well as adults who are eager to explore the science of nature as a life-long hobby. To get the most from the experiments, we recommend using this guide in conjunction with a standard biology text, such as the freely downloadable CK-12 Biology (ck-12.org).

Master the use of the microscope, including sectioning and staining
Build and observe microcosms, soda-bottle worlds of pond life
Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA
Extract, isolate, and observe DNA
Explore photosynthesis, osmosis, nitrogen fixation, and other life processes
Investigate the cell cycle (mitosis and cytokinesis)
Observe populations and ecosystems, and perform air and water pollution tests
Investigate genetics and inheritance
Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection
Gain hands-on lab experience to prepare for the AP Biology exam
https://www.thehomescientist.com/manuals/Illustrated_Guide_to_Home_Biology_Experiments.pdf

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pht
 pht      13.06.2019 - 12:22:47 [7K] , level: 1, UP   NEW
uplne prve helloworld pokusy na agare

vzorka z drazdiaku po 1 dni:
3yOLaQL.jpg

po tyzdni+:
gb0lH9x.jpg

37GmXAh.jpg
aspon 5 roznych kolonii


odtlacok zubnej kefky po 3 dnoch:
ssgn9Uw.jpg

asi vydareny klon bez kontaminacie
4ZRdaW8.jpg

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blba zena
 blba zena      22.07.2019 - 20:28:54 (modif: 22.07.2019 - 20:32:28) [8K] , level: 2, UP   NEW !!CONTENT CHANGED!!
neviem ake medium pouzivas (predpokladam ze variant domaceho LB), ale Drazdiak aj ine priklady mozu byt uplne nevypovedne, lebo kopa mikroorganizmov nie je tak jednoducho kultivovatelna a potrebuju specialnejsie podmienky

niekde by som mala mat pdfko s najcastejsimi mikroorganizmami, ktore pripravil Virologicky ustav na air sampling pri Noci vyskumnikov, zopar veci co ti vyrastlo by si tam urcite identifikoval :)

edit:
air-sampling-popis-Noc-vyskumnikov.jpg

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pht
 pht      23.07.2019 - 13:26:05 , level: 3, UP   NEW
wow dik
pouzil som potato dextrose agar

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jonas
 jonas      23.07.2019 - 11:43:01 , level: 3, UP   NEW
domaci LB nieje zbytocne zlozity? (co pozeram nejake zlozenia)
ja som kupil off-the-shelf malt extract agar s peptonom, ale chcem si robit aj svoj

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blba zena
 blba zena      24.07.2019 - 20:46:56 (modif: 24.07.2019 - 20:53:01), level: 4, UP   NEW !!CONTENT CHANGED!!
to mas to iste:
nejaky extract - malt (v pripade LB yeast)
nejake stiepene bielkoviny, ako zdroj aminokyselin - pepton (v pripade LB tryptone)
agar
dosolis (NaCl), povaris a podavas :)

zavisi co chces kultivovat - pozeram ze ten malt je general na yeast & fungi

aww: https://commons.wikimedia.org/wiki/File:Colony_morphology.svg

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jonas
 jonas      25.07.2019 - 10:17:26 (modif: 25.07.2019 - 10:17:45), level: 5, UP   NEW !!CONTENT CHANGED!!
kebyze to nieje off the shelf tak do toho malt extract agaru davam nutritional yeast. nejaky pepton alebo trypton neviem kde by som zhanal :)
(a hej, klonujem na tom huby)

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pht
 pht      24.07.2019 - 21:02:06 , level: 5, UP   NEW
naco treba dosolit? nie je prave sol antibakterialna?

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blba zena
 blba zena      24.07.2019 - 21:47:17 [1K] , level: 6, UP   NEW
zlozkou media je sol, okrem toho ze sluzi ako zdroj Na/Cl aj kvoli osmotickemu tlaku
https://en.wikipedia.org/wiki/Lysogeny_broth

vysoka koncentracia soli ano (ak sa nebavime o halofiloch)
fyziologicka koncentracia resp. *urcity* rozsah koncentracii pre *urcite* organizmy nie

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pht
 pht      22.07.2019 - 11:43:11 [3K] , level: 2, UP   NEW
drazdiak po dalsom tyzdni:
HGxXVcI.jpg?1

po dychnuti a +1 tyzdni:
j8C0u2Q.jpg

uz zdrha:
Chxy0sX.jpg?1

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jonas
 jonas      22.07.2019 - 14:15:59 (modif: 22.07.2019 - 14:16:11), level: 3, UP   NEW !!CONTENT CHANGED!!
my sme skusili 2 days ago, uz je tam prvy feši

M1TJ6EG.jpg

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milton
 milton      14.06.2019 - 11:42:23 , level: 2, UP   NEW
Na čo sa to pozerám?
Petriho misku poznám. :-)

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pht
 pht      14.06.2019 - 14:35:19 [2K] , level: 3, UP   NEW
neviem na co sa ty pozeras, ale na obrazku su nejake random neidentifikovane bakterialne kolonie a mycelium z kusku hypsizygus tessellatus

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lakerko
 lakerko      14.06.2019 - 08:40:31 , level: 2, UP   NEW
TO POSLEDNE VYZERA AKO PENIS S VAJCAAAAMI!!!

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lazyShepherd
 lazyShepherd      14.06.2019 - 07:45:57 , level: 2, UP   NEW
O co ide? :) Teda ... napises viac?

~ What a time to be alive... ~

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pht
 pht      14.06.2019 - 14:47:36 , level: 3, UP   NEW
ide len o zvedavost, prve pokusy ako narabat s miskami, pri tom drazdiaku a kefke to bolo jedno, ale napriklad pri klonovani huby z obchodu sa kontaminovalo 3 zo 4 misiek aj tak ze som dokladne dezinfikoval a robil v still air boxe

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darmozrac
 darmozrac      15.06.2019 - 07:18:02 , level: 4, UP   NEW
myslim ze na taketo veci by bol lepsi flow box (laminar), videl som aj nejake tutorialy ako si to doma vyrobit

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pht
 pht      15.06.2019 - 09:30:38 , level: 5, UP   NEW
viem pozeral som a zvazujem ale kvoli hepa filtru je to radovo nakladnejsie aj diy, SAB ma vysiel <10e a hodinu casu, da sa s tym asi tiez len to nie je ideal

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stamina
 stamina      14.06.2019 - 00:41:08 [10K] , level: 2, UP   NEW
uz ta vidim, jak to zeres bez chleba!

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pht
 pht      10.06.2019 - 18:04:40 [1K] , level: 1, UP   NEW
https://www.appropedia.org/Hackteria
https://www.hackteria.org/wiki/Main_Page

Hackteria | Open Source Biological Art, DIY Biology, Generic Laboratory Infrastructure

Hackteria is an international network active since 2009 in the field of Open Source Biological Art. As a community platform hackteria tries to encourage the collaboration of scientists, hackers and artists to combine their experitise, write critical and theoretical reflections, share simple instructions to work with lifescience technologies and cooperate on the organization of workshops, temporary labs, hack-sprints and meetings. Hackteria is a network of people practicing DIY (do-it-yourself) and DIWO (do-it-with-others) biology with an interest in art, design and interdisciplinary cooperation. Hackteria operates on a global scale, and is based on a web platform and a wiki for sharing knowledge, which enable anyone to learn but also test different ways of hacking living systems. Hackteria is not based in a physical space, and its goal is to allow artists, scientists and hackers to collaborate and test various biohacking and bioart techniques outside the official laboratories and art institutions, basically anywhere in the world.

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jonas
 jonas      11.06.2019 - 12:54:45 , level: 2, UP   NEW
akurat som vcera pozeral toto: http://hackteria.org/wiki/index.php/Algae_Culture_at_Home
cisticky vzduchu <3

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pht
 pht      05.06.2019 - 16:33:54 [1K] , level: 1, UP   NEW
1*RLrER75EWAIUsrsoNosLGQ.jpeg

A Guide to DIYbio (updated 2019)

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pht
 pht      05.06.2019 - 16:31:45 , level: 1, UP   NEW
https://diybio.org/
https://sphere.diybio.org/

DIYbio.org was founded in 2008 with the mission of establishing a vibrant, productive and safe community of DIY biologists. Central to our mission is the belief that biotechnology and greater public understanding about it has the potential to benefit everyone.